Titlebook: NADPH Oxidases; Methods and Protocol Ulla G. Knaus,Thomas L. Leto Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2

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Spectroscopy of NOX Protein Family Membersulticomponent oxidase complexes. Referred to as cytochrome b558, because of its signature spectral absorbance at 558 nm in reduced-minus-oxidized difference spectroscopy, or cytochrome b(-245), because of its very low midpoint potential of −245 mV at pH 7.0, the protein possesses two stacked inequiv

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Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidaseshe human family, the three oxidases NOX2, NOX1, and NOX3 form a heterodimer with p22. and are regulated by soluble regulatory proteins: p47., its related organizer NOXO1; p67., its related activator NOXA1; p40.; and the small GTPase Rac. Activation of the phagocyte oxidase NOX2 requires p47., p67.,

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Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assaysof this family of enzymes. These model systems have been used to analyze the biosynthesis and functional domains of NOX enzyme components as well as their regulation and cellular activities. This chapter provides an overview of the basic principles and applications of heterologous whole cell assays

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Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCRes, animal models, and targeted drug design. Two basic methods, immunoblotting and reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), are important to monitor NOX protein and messenger RNA (mRNA) levels, respectively, for a range of investigations from understanding cell signali

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Visualization of Intracellular Hydrogen Peroxide with the Genetically Encoded Fluorescent Probe HyPemolecules in a pathological process known as oxidative stress. The HyPer family of genetically encoded probes are a useful noninvasive tool for monitoring the real-time dynamics of ROS in individual cells or model organisms. HyPer, the first genetically encoded probe for detection of hydrogen peroxi

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