| 书目名称 | Multiprotein Complexes |
| 副标题 | Methods and Protocol |
| 编辑 | Arnaud Poterszman |
| 视频video | |
| 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination—x-ray crystallography and cryo-electron microscopy—by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successful.Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protoco |
| 出版日期 | Book 2021 |
| 关键词 | structural biology; X-ray crystallography; nuclear magnetic resonance; cryo-electron microscopy; FRET |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-1-0716-1126-5 |
| isbn_softcover | 978-1-0716-1128-9 |
| isbn_ebook | 978-1-0716-1126-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Springer Science+Business Media, LLC, part of Springer Nature 2021 |
发表于 2025-3-21 16:11:40
