| 书目名称 | Morpholino Oligomers | | 副标题 | Methods and Protocol | | 编辑 | Hong M. Moulton,Jon D. Moulton | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin regeneration, methods for determining to structure of gene networks during development, electroporation, bacterial knockdowns, pretargeting, diagnostic applications of Morpholinos, techniques for delivering Morpholinos .in utero. to developing embryos, and methods offering rapid, hands-free, label-free and inexpensive assays of Morpholino concentrations in biological extracts.. .Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Morpholino Oligomers: Methods and Protocols aims to provide a diverse application of Morpholinos along with protocols that will assist new labs in moving the frontier.. | | 出版日期 | Book 2017 | | 关键词 | modulating microRNA; Morpholinos; fin regeneration; Electroporation; Giardia lamblia | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-6817-6 | | isbn_softcover | 978-1-4939-8307-0 | | isbn_ebook | 978-1-4939-6817-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media LLC 2017 |
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发表于 2025-3-21 17:42:16
