书目名称 | Monoclonal Antibodies | 副标题 | Methods and Protocol | 编辑 | Vincent Ossipow,Nicolas Fischer | 视频video | | 概述 | Offers modern approaches to generate high quality monoclonals.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes sup | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Monoclonal Antibodies: Methods and Protocols., .Second Edition. expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Monoclonal Antibodies: Methods and Protocols, Second Edition. provides crucial initial steps | 出版日期 | Book 2014Latest edition | 关键词 | aggregated monoclonals; antigen generation; crystallization chaperoning; downstream purification of Ig | 版次 | 2 | doi | https://doi.org/10.1007/978-1-62703-992-5 | isbn_softcover | 978-1-4939-6322-5 | isbn_ebook | 978-1-62703-992-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, New York 2014 |
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