书目名称 | Microtubule Dynamics | 副标题 | Methods and Protocol | 编辑 | Anne Straube | 视频video | | 概述 | Presents well-established and newly emerging techniques as performed by seasoned experts in the field.Provides easy to follow instruction written in a clear, step-by-step manner.Includes key tips and | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and back. In .Microtubule Dynamics: Methods and Protocols., experts in the field provide an up-to-date collection of methods and approaches that are used to investigate microtubule dynamics .in vitro. and in cells. Beginning with the question of how to analyze microtubule dynamics, the volume continues with detailed descriptions of how to isolate tubulin from different sources and with different posttranslational modifications, methods used to study microtubule dynamics and microtubule interactions in vitro, techniques to investigate the ultrastructure of microtubules and associated proteins, assays to study microtubule nucleation, turnover, and force production in cells, as well as approaches to isolate novel microtubule-associated proteins and their interacting proteins. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-s | 出版日期 | Book 2011 | 关键词 | Cellular assays; Dynamic instability; Interacting proteins; Microtubule-mediated processes; Regulation; S | 版次 | 1 | doi | https://doi.org/10.1007/978-1-61779-252-6 | isbn_softcover | 978-1-4939-6185-6 | isbn_ebook | 978-1-61779-252-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2011 |
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