Titlebook: Micropropagation Methods in Temporary Immersion Systems; Marco A. Ramírez-Mosqueda,Carlos A. Cruz-Cruz Book 2024 The Editor(s) (if applica

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Large-Scale Micropropagation of Vanilla (, Jacks.) in a Temporary Immersion Bioreactor (TIB) In addition, the use of temporary immersion systems will allow commercial scale-up and the establishment of biofactories dedicated to in vitro vanilla propagation. This chapter describes a large-scale micropropagation protocol for vanilla using temporary immersion bioreactors (TIB).

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BioMINT: A Temporary Immersion System for Agave Micropropagationic interest. However, it is necessary to implement systems that lower production costs without losing the quality of the plantlets obtained. This chapter describes the BioMINT™ bioreactor as an alternative for the micropropagation of agaves in the different stages of the micropropagation process.

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Micropropagation in a RITA, Temporary Immersion Systemin meeting the growing demand for alstroemeria plants in the market. This chapter describes a specific protocol for temporary immersion bioreactor micropropagation of the “Albatroz” cultivar, with the potential for large-scale automation.

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Direct Shoot From Root and True-to-Type Micropropagation of , “Misty Blue” in Partially Immersed Culls needed for flower production. This is the first protocol to describe direct shoot organogenesis from the roots in a liquid half-component of Murashige and Skoog’s (1962) (MS) basal medium supplemented with 1.6 μM NAA and 1.1 μM BA. The regenerated shoots are multiplied and rooted at the same time