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Titlebook: Microbial Systems Biology; Methods and Protocol Ali Navid Book 2022Latest edition Springer Science+Business Media, LLC, part of Springer Na

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FISHing on a Budget,ession in single cells. Many techniques exist to identify and localize transcripts inside the cell, but often they are costly and labor intensive. Here we present a method to use a singly labeled short DNA oligo probe to perform FISH in yeast cells. This method is effective for highly constrained FI
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NanoSIP: NanoSIMS Applications for Microbial Biology,try and is broadly used to decipher ecophysiological traits of environmental microorganisms, metabolic processes in plant and animal tissues, and cross-kingdom symbioses. When combined with stable isotope-labeling—an approach we refer to as .nanoSIMS imaging offers a distinctive means to quantify ne
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Complex Network Analysis in Microbial Systems: Theory and Examples,hole. Network analysis is an approach that is uniquely suited to uncover patterns and organizing principles in a wide variety of complex systems. In this chapter, we will give a detailed description of basic concepts for characterizing empirical networks, frequently used random network models, and h
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Prokaryotic Genome Annotation,as led to an exponential increase in the number of bacterial and archaeal genome sequences available, as well as corresponding increase of genome assembly and annotation tools developed. Together, these hardware and software technologies have given scientists unprecedented options to study their cho
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Metabolic Modeling with MetaFlux,four modes of operation: (1) solving mode executes an FBA model for an individual organism or for an organism community, (2) gene knockout mode executes an FBA model with one or many gene knockouts, (3) development mode assists the user in creating and improving FBA models, and (4) flux variability
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