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Titlebook: Microbial Production; From Genome Design t Hideharu Anazawa,Sakayu Shimizu Book 2014 Springer Japan 2014 Minimum genome factory.Molecular b

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楼主: 管玄乐团
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Hiromichi Kumagai,Mayumi Sasaki,Alimjan Idiris,Hideki Tohda
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Creation of Novel Technologies for Extracellular Protein Production Toward the Development of , Genoinine degradation, was found to contribute to the improvement of enzyme production in strain MGB874. The present study indicated that our results demonstrated the effectiveness of a synthetic genomic approach with reduction of genome size to generate novel and useful bacteria for industrial uses. Fu
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Efficient and Accurate Production of De Novo Designed Large-Size Gene Clusters by a Novel ,-Based Sy DNA fragments are trade-offs in solution and are thus rarely conducted for a single ligation. In contrast, our novel DNA cloning system using . strain 168 as a final host is offered to solve all plausible stumbling blocks in gene assembly technologies. We call the new system BGM (. gene/genome mani
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Application Methodology of Whole Omics Informationpolyketides and non-ribosomal peptide. Comparative genomics of filamentous fungi indicated enrichment of SMB genes on non-syntenic blocks (NSBs) and suggested large number of SMB gene clusters without the core genes remaining unaddressed. Combining computational analyses of omics information using n
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Insights into Metabolism and the Galactose Recognition System from Microarray Analysis in the Fissioe utilization were found to be highly expressed in the mutant. Although galactose residues are not essential for growth of ., galactosylation of protein is required for maintenance of normal cell shape, tolerance toward various drugs, and nonsexual flocculation. We identified fission yeast .., encod
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Multi-enzymatic Systems for the Production of Chiral Compoundsbacterial ω-transaminases capable of catalyzing a stereoselective transamination between a ketone and amine were discovered by enrichment culturing. An (.)-specific ω-transaminase was isolated from a microorganism and characterized, and the relevant gene was cloned. Addition of lactate dehydrogenase
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Approaches for Improving Protein Production by Cell Surface Engineering amounts in . mutants led to the stabilization of autolysins, making the cells more prone to lysis (Kodama et al., J Biosci Bioeng 103:13–21, 2007). In ., we describe the improvement process for protein production from the aspect of prevention of cell lysis.
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