Titlebook: Microbial Environmental Genomics (MEG); Francis Martin,Stephane Uroz Book 2023Latest edition The Editor(s) (if applicable) and The Author(

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Characterization of Seed Mycobiota Using Culture-Dependent and Culture-Independent Approaches,remain limited. While culture-dependent methods have been used to characterize some members of the seed mycobiota, recent culture-independent studies have improved the ease in identifying and characterizing full seed fungal communities. In this chapter, we describe how to survey seed fungi using bot

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Tissue Cultivation, Preparation, and Extraction of High Molecular Weight DNA for Single-Molecule Geicated by co-precipitation of secondary metabolites, the most destructive being polysaccharides, polyphenols, and melanin. Different DNA extraction protocols and clean-up methods have been developed to address challenging materials and contaminants; however, the method of fungal cultivation and tiss

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Analysis of Ancient Microbial DNA,ient human skeletal remains have revolutionized our understanding of human evolution. This research led to the discovery of a new hominin lineage, and demonstrated multiple admixture events with more distantly related archaic human populations such as Neandertals and Denisovans over the last 100,000

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Whole-Genome Sequencing of Bacterial Endophytes From Fresh and Preserved Plant Specimens,host development and defense, but are often not amenable to culture. Gaining insight into the functions of these obligate endophytic bacteria hinges on culture-independent . approaches, which have seen tremendous development in recent years. We describe in this chapter a set of protocols for the ext

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Investigating the Bacterial and Fungal Communities Involved in Dead Biomass Degradation in Forest Sion with high-throughput sequencing, SIP allows for the identification of microbial community members involved in a particular decomposition process. Further information can be gained (in SIP experiments) through gene-targeted metagenomics and metatranscriptomics, opening the possibility to describe

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Ankylo-

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High-Throughput Screening of Fosmid Libraries for Increased Identification of Novel N-Acyl Homoserio construct metagenomic library to discover new N-acyl homoserine lactone (AHL) degrading enzymes based on a direct method or an indirect enrichment procedure. Applicable to any bacterial ecosystem, it enables rapid identification of functional enzymes effective to degrade AHLs.

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Analyzing Prokaryotic Transcriptomics in the Light of Genome Data with the MicroScope Platform,transforms our understanding of thousands of microbial species occurring in our environment. However, despite the development of powerful bioinformatics approaches, full interpretation of the content of these genomes remains a difficult task. To address this challenge, the MicroScope platform has be