| 书目名称 | Microautoradiography and Electron Probe Analysis | | 副标题 | Their Application to | | 编辑 | W. O. Abel,P. Dörmer,J. D. Pickett-Heaps,U. Lüttge | | 视频video | | | 图书封面 |  | | 描述 | In biological literature, several definitions of quantitative autoradio graphy are given. The term is defined as either the determination and com parison of the density of silver grains above various structures or under varying conditions, or the determination of absolute quantities of radio activity. In both these cases, photometric measurement serves for more rapid and more exact evaluation of grain densities than would be possible by visual counting of the grains. The equipment generally used for the photometric measurement of silver grains consists of a microscope, a photocell, an electronic amplifier system and a display unit. Grains can be made accessible to photometric evaluation by various kinds of microscopic illumination: 1. Substage bright-field illumination. 2. Substage dark-field illumination. 3. Incident dark-field illumination. 4. Vertical bright-field illumination. With all these types of illumination, the relationship between the luminous flux I absorbed by the film, scattered into the objective and reflected or diffracted, and the flux 10 which is not affected by the film is used as a measure of grain density. Since these are differential measurements, the ligh | | 出版日期 | Book 1972 | | 关键词 | Autoradiographie; Elektronenstrahlmikroanalyse; Pflanzenphysiologie; Plant physiology; physiology | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-642-87496-3 | | isbn_softcover | 978-3-540-05950-9 | | isbn_ebook | 978-3-642-87496-3 | | copyright | Springer-Verlag Berlin · Heidelberg 1972 |
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发表于 2025-3-21 18:06:00
