Titlebook: Microarray Analysis of the Physical Genome; Methods and Protocol Jonathan R. Pollack Book 2009 Humana Press 2009 BAC.Cancer genomes.Cytosin

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Methylation Analysis by DNA Immunoprecipitation (MeDIP),e analyzed in a locus-specific manner using PCR assay or in a genome-wide fashion by comparative genomic hybridization against a sample without MeDIP enrichment. This article describes the detailed protocol for MeDIP and hybridization of MeDIP DNA to a whole-genome tiling path BAC array.

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Combining Chromatin Immunoprecipitation and Oligonucleotide Tiling Arrays (ChIP-Chip) for Functionas an unbiased assessment of the potential biological function of any DNA associated factor or epigenomic mark. Through integration of ChIP-chip data with complementary genome-wide approaches including expression profiling, CGH and SNP arrays, novel paradigms of transcriptional regulation and chromatin structure are emerging.

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