Titlebook: MicroRNA Technologies; Min Jeong Kye Book 2017 Springer Science+Business Media LLC 2017 C.elegans.cellular differentiation.eukaryotic cell

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Book 2017 nervous systems. .MicroRNA Technologies .also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression

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MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and T and dual luciferase assay. Despite the inherent differences between cultures, we were able to consistently show that 3D topography promotes differentiation of hNSCs through modulation of miRNAs associated with cell proliferation and maintenance of stemness.

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Isolating and Screening Subcellular miRNAs in Neuron,r distribution in neuron varies. This suggests that there are cellular mechanisms actively regulating miRNA expression and localization in subcellular compartments of neurons. In this chapter, we review currently available methods of synaptic miRNA profiling from isolating samples, purifying RNAs, and measuring expression.

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Measuring miRNA Mediated Translational Regulation with Live Cell Imaging,ry after photobleaching (FRAP) has been widely used to measure local protein synthesis rate in axons. This technique allows us to measure speed and efficiency of translation of certain mRNAs. We modified this method to measure how miRNAs influence on local synthesis of proteins in neurons.

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Protocol for miRNA In Situ Hybridization on Mouse Spinal Cord,specific cell types sheds light on differential regulation of their expression and on the potential functional roles of miRNAs in the brain. In this chapter, we present a simple and robust protocol for the detection of miRNAs by in situ hybridization in formalin-fixed paraffin-embedded spinal cords of mature mice and developing embryos.

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0893-2336 tion advice from the experts.This volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. .MicroRNA Technologies .also explores newly established techniques to study the role of miRNAs in the ne

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