Titlebook: Methods of Protein Microcharacterization; A Practical Handbook John E. Shively Book 1986 Springer Science+Business Media LLC 1986 amino aci

penance

Anemia

Infantry

提名的名单

Analysis of Phenylthiohydantoin Amino Acids by HPLC. It is the only technique commonly used for PTH analysis that can resolve all of the PTH amino acids in a single chromatographic run. Because it provides quantitative data at the pmol level, HPLC is also the only available method suitable for microsequencing that uses the new generation of automated Edman sequencers (.,.).

Interregnum

initiate

Manual Edman Sequencing Systementific discipline, existing as it does merely to supply probes to the molecular geneticists and occasionally to check their DNA reading frames, and one has a method in deep psychological crisis. Fortunately, manual sequencing can provide any of the structural data that requires or advantageously utilizes protein chemistry.

宽大

Gas-Phase Protein/Peptide Sequencer). The resulting amino acid derivative-an anilinothiazolinone (ATZ)- is converted to the more stable phenylthiohydantoin (PTH) amino acid, which is analyzed by high performance liquid chromatography (HPLC). The remaining peptide is subjected to further degradation cycles to generate its amino acid sequence.

Genome

Design of a Multipurpose Sequencer-called liquid-phase technique, in which the protein is degraded as thin film in a spinning cup, has been widely used and successfully applied to the sequence determination of proteins and polypeptide fragments.

nitroglycerin

先驱

COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methodsrminal modifications, especially for many regulatory pep-tides (.,.). Thus, whenever Edman degradation is used as the prinicpal method of complete amino acid sequence determination, it must be supplemented by COOH-terminal analysis.