Titlebook: Methods of Hybridoma Formation; Arie H. Bartal,Yashar Hirshaut Book 1987 The Humana Press Inc. 1987 DNA.Expression.Interferon.Macrophages.

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Antibody-Secreting Human-Human Hybridomas,blishment of long-term B-cell cultures of non-transformed cells has met with very limited success (1, 2), whereas somatic cell hybridization techniques have been used for years to immortalize cells and were successfully applied to immortalize Ig-secreting mouse B-lymphocytes, resulting in murine hyb

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Generation of Mutant Monoclonal Antibodies,cine. Initially it seemed sufficient to obtain a monoclonal antibody with the desired specificity. However, it soon became obvious that many of the available monoclonal antibodies were not optimally effective for the task to which they were being applied. In order to illustrate this, and present som

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Generation of Mutant Monoclonal Antibodies,e of the solutions to these problems, we will first briefly describe the relevant structural aspects of the immunoglobulin molecule, then review the kinds of problems that have arisen, and finally present in some detail some solutions to these problems.

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Mechanisms of Cell Fusion and Selection in the Generation of Hybridomas,owed for the development of the hybridoma technology will be considered. In addition, some recent advances in cell hybridization and selection procedures that may be useful in enhancing the production of hybridomas will be discussed.

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Use of Heteromyelomas in the Enhancement of Human Monoclonal Antibody Production,of mouse myeloma cells with spleen cells from an immunized mouse. The ready availability of murine monoclonal antibodies as biological probes has changed the approaches in many diagnostic and therapeutic areas of medicine.