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Titlebook: Methods in Non-Aqueous Enzymology; Munishwar Nath Gupta Book 2000 Springer Basel AG 2000 biochemistry.biotechnology.carbohydrates.catalysi

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书目名称Methods in Non-Aqueous Enzymology
编辑Munishwar Nath Gupta
视频video
丛书名称Methods and Tools in Biosciences and Medicine
图书封面Titlebook: Methods in Non-Aqueous Enzymology;  Munishwar Nath Gupta Book 2000 Springer Basel AG 2000 biochemistry.biotechnology.carbohydrates.catalysi
描述Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac­ tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol­ ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol­ ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac­ tivity and stabilization via immobilization. Some s
出版日期Book 2000
关键词biochemistry; biotechnology; carbohydrates; catalysis; enzyme; enzymes; enzymology; Lipid; lipids; Peptid; pre
版次1
doihttps://doi.org/10.1007/978-3-0348-8472-3
isbn_softcover978-3-7643-6109-9
isbn_ebook978-3-0348-8472-3
copyrightSpringer Basel AG 2000
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Strategies for Improving the Lipase-Catalyzed Preparation of Chiral Compounds,state analogs. It was found that in most lipases, a helical lid covers the active site thus blocking access of substrates. Upon binding to a hydrophobic interface such as a lipid droplet, the lid opens enabling substrates access to the active site thus increasing the catalytic activity of lipases. S
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Analysis in Non-Aqueous Milieu Using Thermistors,ased analysis in organic solvents. In the past decade, however, it has been demonstrated by several workers that enzymes can function effectively well in organic phase as well as in aqueous phase [.]. In this regard an early work [.] in organic phase biosensors followed by other investigations on en
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Importance of the Medium for , and , Protein Folding Mechanisms: Biomedical Implications,teins will subsequently lead to the build-up of aggregates either in living tissue . or in bioprocessing solutions . The build-up of aggregates in living tissue has been identified as a cause for many intractable diseases such as Alzheimer’s. It is these mis-folded proteins that give rise to senile
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further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac­ tivity and stabilization via immobilization. Some s978-3-7643-6109-9978-3-0348-8472-3
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age drinking..- Family-, school-, and community-based prevention programs..- Treatment of comorbid substance and psychiatric disorders..Clinicians, researchers, and policy makers will find this a bedrock source of evidence-based knowledge, whether one’s goal is choosing an age-appropriate assessment
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