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Titlebook: Methods in Membrane Lipids; Dylan M. Owen Book 2015Latest edition Springer Science+Business Media New York 2015 Cell membranes.X-Ray scatt

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发表于 2025-3-21 19:16:41 | 显示全部楼层 |阅读模式
书目名称Methods in Membrane Lipids
编辑Dylan M. Owen
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Methods in Membrane Lipids;  Dylan M. Owen Book 2015Latest edition Springer Science+Business Media New York 2015 Cell membranes.X-Ray scatt
描述.This volume expands upon the previous edition with current, detailed protocols for investigating membranes and their component lipids in artificial membranes, cells, and .in silico.. Chapters focus on properties of the component lipids, membranes and their biophysical properties, fluorescent probes for studying membranes, sample preparation, physical techniques to study membrane composition, properties , and function, behavior of cholesterol within a bilayer and examination of cholesterol-dependent phase separation. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical, .Methods in Membrane Lipids, Second Edition .seeks to aid scientist in further study into membrane lipids..
出版日期Book 2015Latest edition
关键词Cell membranes; X-Ray scattering; archetypes; artificial membranes; asymmetric planar lipid bilayers; ato
版次2
doihttps://doi.org/10.1007/978-1-4939-1752-5
isbn_softcover978-1-4939-5399-8
isbn_ebook978-1-4939-1752-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

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Electron Microscopy Methods for Studying Plasma Membranes,hich these distributions are clustered or dispersed and allows the extent of co-localization between different cell surface components to be precisely determined. This approach has been successfully applied to the study of signalling network organization and the consequences of physiological changes in modulating cell surface function.
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X-Ray Diffraction of Lipid Model Membranes,y scattering (WAXS), are discussed. Methods by which the sign of the interfacial curvature of non-lamellar phases may be determined are then presented. Finally, the calculation of electron density profiles from the intensities of the observed Bragg peaks is described, for the lamellar phase and for the inverse hexagonal phase.
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A Comparative LC-MS Based Profiling Approach to Analyze Lipid Composition in Tissue Culture Systemsocessing steps to compare the global lipid profiles and identify species that are enhanced or depleted. Identifying lipids that change is the first step towards functional studies to unravel their roles.
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Solid State NMR of Lipid Model Membranes,date both the structure and dynamics over a wide variety of timescales. This coupled with a non-perturbing label .H is able to provide information such as the order parameters for a wide variety of different liquid phases.
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