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Titlebook: Methods in Endothelial Cell Biology; Hellmut G. Augustin Book 2004 Springer-Verlag Berlin Heidelberg 2004 Angiogenesis.Cell culture.Vascul

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书目名称Methods in Endothelial Cell Biology
编辑Hellmut G. Augustin
视频video
概述A comprehensive collection of reliable practical protocols.All step-by-step assays are suited for scientists and students with differing technical expertise.With a Foreword by Judah Folkman, Harvard M
丛书名称Springer Lab Manuals
图书封面Titlebook: Methods in Endothelial Cell Biology;  Hellmut G. Augustin Book 2004 Springer-Verlag Berlin Heidelberg 2004 Angiogenesis.Cell culture.Vascul
描述.Endothelial cell biology has developed into a vibrant discipline and has become a critical instrument to study several disease processes on the cellular and molecular level. It is now widely recognized that dysfunctions of normal endothelial cell homeostasis are involved in some of the most important human diseases, including ischemic heart diseases, hypertension, atherosclerosis, tumors, diabetes, arthritis, and inflammation. Further, the increasing importance and recognition of the field of vascular biology in general requires in vitro and in vivo techniques in order to address the complex questions...Methods in Endothelial Cell Biology. is a comprehensive practical "how-to"-guide summarizing the most relevant established techniques as well as a number of new emerging techniques. Easy-to-follow reliable protocols provide a useful lab bench resource for the experienced researcher and newcomer to the field. .
出版日期Book 2004
关键词Angiogenesis; Cell culture; Vascular Biology; Vivo; blood vessel; bone; cell biology; endothelium; gene tran
版次1
doihttps://doi.org/10.1007/978-3-642-18725-4
isbn_softcover978-3-540-21397-0
isbn_ebook978-3-642-18725-4
copyrightSpringer-Verlag Berlin Heidelberg 2004
The information of publication is updating

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Isolation,Culture, and Differentiation of Circulating Endothelial Precursor Cellsutes to postnatal neovascularization offers novel therapeutic strategies for the use of ex vivo cultured circulating endothelial progenitor cells or their precursors for cell therapy of tissue ischemia.
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Three-Dimensional Spheroid Culture of Endothelial Cells example, CD34, which is constitutively expressed by most EC in vivo, is rapidly downregulated upon transfer into tissue culture (Delia et al. 1993). Similarly, cultured brain EC lose their tight-junction-dependent blood-brain barrier characteristics (Wolburg et al. 1994).
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Immortalization of Endothelial Cellsection with calcium phosphate or lipid-based methods substantially add to the inconvenience. Permanently growing endothelial cell lines that have been immortalized by the action of dominant oncogenes can provide an elegant solution to these limitations that are hampering the analysis of endothelial cells.
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Detection, Isolation and Culture of Lymphatic Endothelial Cellslecular biological studies, and these were mainly derived from lymphatic tumors. However, the identification of lymphatic specific markers during the past few years and the isolation and maintenance of primary cultures of lymphatic endothelial cells have enabled studies of the molecular properties of these cells.
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