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Titlebook: Methods in DNA Amplification; Arndt Rolfs,Ines Weber-Rolfs,Ulrich Finckh Book 1994 Springer Science+Business Media New York 1994 Hepatitis

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书目名称Methods in DNA Amplification
编辑Arndt Rolfs,Ines Weber-Rolfs,Ulrich Finckh
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图书封面Titlebook: Methods in DNA Amplification;  Arndt Rolfs,Ines Weber-Rolfs,Ulrich Finckh Book 1994 Springer Science+Business Media New York 1994 Hepatitis
描述The polymerase chain reaction (PCR) - an in Vitro techniques for producing large amounts of a specific DNA fragment - has rapidly become established as one of the most important, impressive and fascinating methods of molecular biology as well as clinical diagnostics. In the seven years since‘the technique was published, it has had a major impact on medical research. However, as there are still problems in instruments, standardized protocols for diagnostic applications and unsolved difficulties to avoid cross-contaminations on the one hand and on the other hand the even present question of how to interpret the biological value of a PCR­ result, most clinicians prefer to further wait until these topics are clarified. It is the aim of this book to give the reader lab-proven protocols from experienced scientists as well as a general introduction to alternative DNA-amplification procedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rap
出版日期Book 1994
关键词Hepatitis; Listeria monocytogenes; Virus; bacteria; infectious; leukocytes; tuberculosis; infectious diseas
版次1
doihttps://doi.org/10.1007/978-1-4615-2530-1
isbn_softcover978-1-4613-6078-0
isbn_ebook978-1-4615-2530-1
copyrightSpringer Science+Business Media New York 1994
The information of publication is updating

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Qualitative and Quantitative Detection of Nucleic Acids of Infectious Agents by NASBAence (figure 1). Although capable of amplifying both DNA and RNA target sequences, NASBA. is most suitable for the amplification of RNA. Thus, NASBA. has become an extremely powerful technique for the detection and quantification of retroviruses (particularly HIV-1).
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Confirmation of , Carrier State by Polymerase Chain Reaction in Cases with Nondiagnostic Serologiesiagnostic results in the different kits were found. The HCV-specific polymerase chain reaction (PCR) technique was used to search for the presence of HCV RNA in these specimens. Interestingly, 6 of 22 serum specimens were found to be HCV-PCR positive. Our results demonstrated that PCR remains the re
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cedures and their possible usage such as the NASBA or LCR. This book is divided into four major parts to provide a theoretical (first and second section) and a practical framework for a better understanding of the new technology. In the first part we provide an up-to-date summary of basic problems in this rap978-1-4613-6078-0978-1-4615-2530-1
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and a test suite of temporal database queries. The Zurich workshop was conceived from the outset to be universal in scope, and international in participation. The Call for Papers sought to evoke the highest quality and most up-to-date temporal database research from around the world. Mindful of the
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D. Myerson. Therapeutic drug monitoring and clinical toxicity are faced by new challenges every day as the problem-solving approaches are fetched with more complex causes in regulatory and health technology to assist the healthcare system. Drug monitoring for the patient is enhancing its functioning by variou
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L. Cross,C. Potts,J. G. Ansoni-channel CNN-LSTM strategy outperforms the other two techniques, achieving a mean absolute percentage error reduction of up to 25%-58%. The potential of utilizing multi-channel data for accurate PHM of batteries can be concluded from the present work.
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