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Titlebook: Methods in Biological Oxidative Stress; Kenneth Hensley,Robert A. Floyd Book 2003 Humana Press 2003

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Fluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biologicrature/acidic conditions to produce a chromogenic/fluorescent product that can be easily measured employing various analytical techniques such as spectrophotometric (.,.–.) or fluorometric methods (.,.–.). Incorporating HPLC with ultraviolet (UV)/fluorometric detection or gas chromatography-mass spe
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HPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitratlipid nitration products in biological matrices primarily as biomarkers to determine the level of oxidative stress and tissue damage. 3-Nitrotyrosine has been determined to be a useful protein biomarker employed to measure oxidative stress based in previous publications (.–.). The most commonly meas
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Isotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Prosyl radical does not generate .-tyrosine and .-tyrosine, however (.–.). Another oxidant, hypochlorous acid (HOCl), produces 3-chlorotyrosine when it reacts with tyrosine (.,.). HOCl is generated only by myeloperoxidase, which requires H.O. and Cl. to perform the reaction. Thus, determining relative
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Detection of Aldehydic DNA Lesions Using Aldehyde Reactive Probe(.), the sugar-phosphate backbone is also highly vulnerable to attack. Abstraction of a hydrogen atom from each carbon from deoxyribose produces a carbon-based sugar radical that can rearrange, resulting in scission of the nucleic acid strand, deoxyribose fragmentation, as well as formation of base
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