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Titlebook: Methods for Affinity-Based Separations of Enzymes and Proteins; Munishwar Nath Gupta Book 2002 Springer Basel AG 2002 antibody.biomolecule

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Expanded Bed Affinity Chromatography,A) technology. Particular attention is given to its use as an affinity separation method that can in most circumstances be used early on in a downstream processing (DSP) scheme. Details of the quantitative methods used to analyze the performance of an EBA operation and scale-up criteria are presented and discussed.
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Cellulose-Binding Fusion Proteins, or polyhistidine tails. As well as mediating specific binding for affinity purification of the fusion partner, the affinity tag can also increase the stability of the product to proteolysis, modulate its solubility and control its cellular location in the expression host.
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Perfusion Affinity Chromatography for Rapid Antibody Separations,ugh-pores allow convective (or “perfusive”) flow through the bead. Along the convective through-pores are smaller diffusive pores with short diffusive path lengths. The combination of convection and diffusion allows perfusion chromatography to be run at extremely high flow rates, in excess of 15,000 cm/h.
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Arvind Lalin regions, and distributive equity is examined. Cross-border policy dialogue has to identify the most binding constraints for new industries at both regional and national levels. This ideal-type policy is juxtaposed against the current practice of ‘buy national’ campaigns in East Africa.
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