Titlebook: Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols; Cutting-Edge Technol Yinduo Ji Book 2020Latest edition Springer Science+Busin

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Identification of Virulence Determinants During Host-Pathogen Interaction Using Tn-Seq Technology,hin specific host environments. In this chapter, we describe the application of Tn-seq to a murine model of osteomyelitis as a paradigm for using this powerful technology to elucidate mechanisms of bacterial pathogenesis.

cushion

Genetic Manipulation of MRSA Using CRISPR/Cas9 Technology, genome editing in ., including genome modification and base editing. This protocol outlines in detail the design of primers, the construction and transformation of editing plasmids, as well as the verification of sequence-specific CRISPR/Cas9-mediated mutagenesis in ..

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gastritis

978-1-4939-9851-7Springer Science+Business Media, LLC, part of Springer Nature 2020

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沙文主义

Yinduo JiIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Genetic Manipulations of Staphylococcal Chromosomal DNA,g temperature-sensitive plasmids to generate mutations in ., including single-nucleotide mutations, insertions, and gene deletions. In addition, this chapter summarizes other key genetic technologies used for the manipulation of ., including the CRISPR/Cas9 system and complementation.

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Ting Lei,Junshu Yang,Aaron Becker,Yinduo Jiuum sucking and pulling, and performance probability distribution. Highlighting outstanding innovations and reflecting the latest advances in intelligent agricultural equipment in China, the book offers a uniqu978-981-16-1286-2978-981-16-1284-8Series ISSN 2195-9862 Series E-ISSN 2195-9870