Titlebook: Metastasis Research Protocols; Miriam Dwek,Susan A. Brooks,Udo Schumacher Book 2012Latest edition Springer Science+Business Media, LLC 201

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Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells,y employed across different laboratories, however, renders comparison of results difficult. This chapter describes a robust reliable system for the immunochemical detection of individual micrometastatic tumour cells from the BM aspirates of patients with solid epithelial tumours, based on the binding of antibody to cytokeratin proteins.

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https://doi.org/10.1007/978-1-61779-854-2Metastasis; RNAi technology; SDS-PAGE; affinity measurement of biomolecular interactions; cancer cells; c

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Book 2012Latest edition second edition of .Metastasis Research Protocols, .brings together the most up to date versions of the seminal techniques that were presented in the first edition and also includes new techniques that have recently been shown to be important in illuminating the processes underlying this important a

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2-DE-Based Proteomics for the Analysis of Metastasis-Associated Proteins, 2-DE. Guidelines for the preparation and storage of buffers, experimental conditions and protocols of affinity chromatography, isoelectric focussing, and SDS-PAGE conditions are provided. Critical parameters associated with the different steps of 2-DE are discussed.

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RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Moleselection, single-cell dilution, and subsequent FACS analysis. The cell line showed a knock-down of CEACAM1 of more than 85%. This knock-down remained stable when examined in an SCID mouse xenograft experiment over 40 days.

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Lectin Array-Based Strategies for Identifying Metastasis-Associated Changes in Glycosylation,es in glycosylation. This chapter describes the generation of a lectin-binding signature associated with metastatic primary breast tumours that have been resected, fixed, and embedded in paraffin. Procedures concerning sample and lectin microarray preparation are explained, alongside experimental considerations and approaches to data analysis.

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The Polymerase Chain Reaction,thermostable DNA polymerase ensure that abundant copies are reliably reproduced to levels that can be visualized and/or used for further applications. This chapter describes applications of PCR and PCR-RT to investigate primary cancer and metastatic disease at both the DNA and mRNA expression levels.