Titlebook: Metals in the Brain; Measurement and Imag Anthony R. White Book 2017 Springer Science+Business Media LLC 2017 Microscopy.fixation artifacts

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Measuring Changes in Brain Manganese or Iron Using Magnetic Resonance Imaging (MRI),centrations in vivo in animal models of brain disease. Here, we specifically describe how to measure changes in Mn in the brain from qMRI measurements of the longitudinal relaxation rate, .., in rats at a magnetic field strength of 7 T. We further discuss how to extend the method to measure changes in Mn or Fe from other qMRI parameters.

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Fluorescent Probes for the Analysis of Labile Metals in Brain Cells,crucial to different types of biological investigations. Finally, we provide a detailed method to prepare, treat, and image cellular models that should be applicable to a diverse range of fluorescent sensors, with suggestions for methods to analyze results using image analysis software.

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0893-2336 ation advice from the experts.This volume explores methods used to examine metal levels and distribution in brain tissue or brain-derived cells. The chapters in this book discuss the use of fluorescent metal probes, synchrotron-based X-ray microscopy, ICP-MS, laser ablation-ICP-MS, laser-based tissu