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Titlebook: Metabolic Signaling; Methods and Protocol Sarah-Maria Fendt,Sophia Y. Lunt Book 2019 Springer Science+Business Media, LLC, part of Springer

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书目名称Metabolic Signaling
副标题Methods and Protocol
编辑Sarah-Maria Fendt,Sophia Y. Lunt
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Metabolic Signaling; Methods and Protocol Sarah-Maria Fendt,Sophia Y. Lunt Book 2019 Springer Science+Business Media, LLC, part of Springer
描述.This book provides protocols to quantify metabolism, to identify metabolic crosstalk, and to setup and develop tools and models to gain insight into metabolic signaling using experimental and computational approaches.  Chapters detail protocols to quantify metabolism, identify metabolic crosstalk, and develop tools and models to gain a systems-level insight into metabolic signaling. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Authoritative and cutting-edge, .Metabolic Signaling: Methods and Protocols. aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level..
出版日期Book 2019
关键词nutrient metabolism; cancer cells; tumors; deuterium tracing; lipectomized mice
版次1
doihttps://doi.org/10.1007/978-1-4939-8769-6
isbn_softcover978-1-4939-9387-1
isbn_ebook978-1-4939-8769-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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Measuring the Nutrient Metabolism of Adherent Cells in Culture,tured adherent mammalian cells to collect both polar and nonpolar intracellular metabolites. This chapter also describes experimental design considerations for performing stable isotope labeling experiments, and the use of chemical derivatization to increase the number of compounds that can be detected using one chromatography method.
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13C Tracer Analysis and Metabolomics in 3D Cultured Cancer Cells,orage-independent growth, spheroid formation, and extracellular matrix production by (cancer) cells. Here, we describe how to perform metabolomics and .C tracer analysis in 3D cultures of cancer cells.
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Book 2019ucible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. . Authoritative and cutting-edge, .Metabolic Signaling: Methods and Protocols. aims to provide researchers with methods to study, perturb, and functionally interpret metabolism and metabolic signaling from the sub-cellular to the whole-body level..
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Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers,e, but also for the identification of anti-angiogenic metabolic targets. This chapter describes in detail the radioactive tracer substrate assays that have been used for the determination of EC metabolic flux in vitro.
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Development of Patient-Derived Tumor Xenograft Models,ed mice, allowing for the progressive growth and amplification of cancer tissue for in vivo testing. Here, we describe the detailed methods we developed to establish PDX from both surgically removed endometrial cancer fragments (endometrial cancer) and fine-needle aspiration biopsies (pancreatic cancer).
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