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Titlebook: Metabolic Flux Analysis in Eukaryotic Cells; Methods and Protocol Deepak Nagrath Book 2020 Springer Science+Business Media, LLC, part of Sp

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书目名称Metabolic Flux Analysis in Eukaryotic Cells
副标题Methods and Protocol
编辑Deepak Nagrath
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Metabolic Flux Analysis in Eukaryotic Cells; Methods and Protocol Deepak Nagrath Book 2020 Springer Science+Business Media, LLC, part of Sp
描述This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Cutting-edge and comprehensive, .Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols. is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases..
出版日期Book 2020
关键词lipid metabolism; Dynamic stable-isotope modeling; transcriptional regulatory networks; pSIRM; Exosome-m
版次1
doihttps://doi.org/10.1007/978-1-0716-0159-4
isbn_softcover978-1-0716-0161-7
isbn_ebook978-1-0716-0159-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
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Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13Cmetabolites significantly add to the number of analytes. In this protocol chapter, we discuss two liquid chromatography methods, namely, reverse phase ion-pairing and hydrophilic interaction chromatography (HILIC) that together can separate a wide variety of metabolites that are typically used for .C metabolic flux analysis.
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Inferring Metabolic Flux from Time-Course Metabolomics,hm, a genome-scale modeling approach that uses time-course metabolomics to predict dynamic flux rewiring during transitions between metabolic states. This chapter provides a protocol for applying DFA to characterize the dynamic metabolic activity of various cancer cell lines.
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Quantifying Nitric Oxide Flux Distributions,ed protocol, which includes experimental measurements and computational modeling, to estimate the NO flux distribution in an . culture. Those fluxes will have uncertainty associated with them and we also discuss how further experiments and modeling can be employed for flux refinement.
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