书目名称 | Metabolic Flux Analysis in Eukaryotic Cells |
副标题 | Methods and Protocol |
编辑 | Deepak Nagrath |
视频video | |
概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts |
丛书名称 | Methods in Molecular Biology |
图书封面 |  |
描述 | This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Cutting-edge and comprehensive, .Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols. is a valuable resourcefor both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.. |
出版日期 | Book 2020 |
关键词 | lipid metabolism; Dynamic stable-isotope modeling; transcriptional regulatory networks; pSIRM; Exosome-m |
版次 | 1 |
doi | https://doi.org/10.1007/978-1-0716-0159-4 |
isbn_softcover | 978-1-0716-0161-7 |
isbn_ebook | 978-1-0716-0159-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
issn_series | 1064-3745 |
copyright | Springer Science+Business Media, LLC, part of Springer Nature 2020 |