书目名称 | Membrane Trafficking | 副标题 | Methods and Protocol | 编辑 | Jingshi Shen | 视频video | | 概述 | Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to .in vitro. biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Membrane Trafficking: Methods and Protocols. provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.. | 出版日期 | Book 2022 | 关键词 | Endomembrane systems; Exocytic pathway; Endocytic pathway; Membrane biology; CRISPR genetic screens | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2209-4 | isbn_softcover | 978-1-0716-2211-7 | isbn_ebook | 978-1-0716-2209-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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