找回密码
 To register

QQ登录

只需一步,快速开始

扫一扫,访问微社区

Titlebook: Membrane Trafficking; Methods and Protocol Jingshi Shen Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license

[复制链接]
查看: 42852|回复: 61
发表于 2025-3-21 18:26:34 | 显示全部楼层 |阅读模式
书目名称Membrane Trafficking
副标题Methods and Protocol
编辑Jingshi Shen
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Membrane Trafficking; Methods and Protocol Jingshi Shen Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license
描述This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to .in vitro. biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Membrane Trafficking: Methods and Protocols. provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs..
出版日期Book 2022
关键词Endomembrane systems; Exocytic pathway; Endocytic pathway; Membrane biology; CRISPR genetic screens
版次1
doihttps://doi.org/10.1007/978-1-0716-2209-4
isbn_softcover978-1-0716-2211-7
isbn_ebook978-1-0716-2209-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

书目名称Membrane Trafficking影响因子(影响力)




书目名称Membrane Trafficking影响因子(影响力)学科排名




书目名称Membrane Trafficking网络公开度




书目名称Membrane Trafficking网络公开度学科排名




书目名称Membrane Trafficking被引频次




书目名称Membrane Trafficking被引频次学科排名




书目名称Membrane Trafficking年度引用




书目名称Membrane Trafficking年度引用学科排名




书目名称Membrane Trafficking读者反馈




书目名称Membrane Trafficking读者反馈学科排名




单选投票, 共有 1 人参与投票
 

0票 0.00%

Perfect with Aesthetics

 

0票 0.00%

Better Implies Difficulty

 

1票 100.00%

Good and Satisfactory

 

0票 0.00%

Adverse Performance

 

0票 0.00%

Disdainful Garbage

您所在的用户组没有投票权限
发表于 2025-3-21 20:23:55 | 显示全部楼层
发表于 2025-3-22 01:24:40 | 显示全部楼层
发表于 2025-3-22 04:34:22 | 显示全部楼层
Membrane Trafficking978-1-0716-2209-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-22 12:36:55 | 显示全部楼层
Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp TechniqueWhole-cell patch clamping is a standard method to monitor the secretion of synaptic vesicles. In this chapter, we describe the basic steps of whole-cell patch clamping for measuring synaptic exocytosis, aiming to provide reference for researchers who are new to this field.
发表于 2025-3-22 14:25:11 | 显示全部楼层
https://doi.org/10.1007/978-1-0716-2209-4Endomembrane systems; Exocytic pathway; Endocytic pathway; Membrane biology; CRISPR genetic screens
发表于 2025-3-22 21:06:03 | 显示全部楼层
Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook scently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).
发表于 2025-3-23 00:11:06 | 显示全部楼层
In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusionoteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.
发表于 2025-3-23 05:17:13 | 显示全部楼层
Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)ws for visualization of membrane dynamics at the cell surface with superb spatiotemporal resolution. In this chapter, TIRFM is used to record and analyze exocytosis of single glucose transporter-4 (GLUT4) containing vesicles in 3T3-L1 adipocytes.
发表于 2025-3-23 06:22:32 | 显示全部楼层
Julia Dancourt,Grégory Lavieuwith detailed examples and exercises at the end of each chap.Inventories are prevalent everywhere in the commercial world, whether it be in retail stores, manufacturing facilities, government stockpile material, Federal Reserve banks, or even your own household. This textbook examines basic mathemat
 关于派博传思  派博传思旗下网站  友情链接
派博传思介绍 公司地理位置 论文服务流程 影响因子官网 SITEMAP 大讲堂 北京大学 Oxford Uni. Harvard Uni.
发展历史沿革 期刊点评 投稿经验总结 SCIENCEGARD IMPACTFACTOR 派博系数 清华大学 Yale Uni. Stanford Uni.
|Archiver|手机版|小黑屋| 派博传思国际 ( 京公网安备110108008328) GMT+8, 2025-5-7 21:43
Copyright © 2001-2015 派博传思   京公网安备110108008328 版权所有 All rights reserved
快速回复 返回顶部 返回列表