| 书目名称 | Membrane Protein Structure and Function Characterization | | 副标题 | Methods and Protocol | | 编辑 | Jean-Jacques Lacapere | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful .Methods in Molecular Biology .series format, chapters in .Membrane Protein Structure andFunction Characterization: Methods and Protocols .provide. .different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics | | 出版日期 | Book 2017 | | 关键词 | membrane protein; folded protein; membrane mimicking environments; functional status; antibodies | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-7151-0 | | isbn_softcover | 978-1-4939-8399-5 | | isbn_ebook | 978-1-4939-7151-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media LLC 2017 |
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发表于 2025-3-21 19:26:42
