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Titlebook: Meiosis; Methods and Protocol Jesús A Carballo Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spring

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发表于 2025-3-21 18:08:10 | 显示全部楼层 |阅读模式
书目名称Meiosis
副标题Methods and Protocol
编辑Jesús A Carballo
视频video
概述Includes cutting-edge methods and protocols.Contains key notes and implementation advice from the experts.Provides step-by-step detail essential for reproducible results
丛书名称Methods in Molecular Biology
图书封面Titlebook: Meiosis; Methods and Protocol Jesús A Carballo Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spring
描述.This volume details protocols on meiosis, covering the latest technological and methodological advances in this research field. Chapters guide readers through methods on genomics, biochemistry, super-resolution microscopy, traditional genetics, cytological methods, as well as machine learning and .in silico. modelling. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Meiosis: Methods and Protocols. aims to ensure successful results in further studies of this vital field..
出版日期Book 2024
关键词proteinaceous structure; mitotic prophase; DNA replications; Cryo-EM; recombination machinery
版次1
doihttps://doi.org/10.1007/978-1-0716-3906-1
isbn_softcover978-1-0716-3908-5
isbn_ebook978-1-0716-3906-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
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发表于 2025-3-21 22:48:30 | 显示全部楼层
Temporal Analysis of DSB Repair Outcome in , Meiosisrs in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to . meiotic DSB repair dynamics in the germline.
发表于 2025-3-22 02:33:10 | 显示全部楼层
Culture of the Intact Postnatal Naked Mole-Rat Ovary: From Meiotic Prophase to Single-Cell RNASeqnew possibilities in the field. In this chapter, we present a comprehensive collection of protocols that permit the culture of whole naked mole-rat ovaries, followed by analysis of germ cells, from PGCs to oocytes, in meiotic prophase I, as well the obtention of single-cell suspension or single-nuclei suspension for RNASeq.
发表于 2025-3-22 07:55:21 | 显示全部楼层
Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in tein to a designated location. Anchor away is a reliable tool for the study of meiotic proteins, as only small quantities of rapamycin are required to efficiently and rapidly translocate the protein of interest without compromising meiotic progression.
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Dynamic Live Cell Imaging of Budding Yeast Meiosis in fluorescent chromosome tagging techniques has made it possible to use yeast more effectively for imaging-based approaches as well. This protocol describes live cell imaging methods that can be used to monitor chromosome movements throughout meiosis in living yeast cells.
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发表于 2025-3-23 00:46:27 | 显示全部楼层
synapsis: A Bioconductor Package to Automate the Analysis of Meiotic Double-Strand Break Repair and fying meiotic nuclei and quantifying key double-strand break formation and repair events in a rapid, scalable, and reproducible workflow, and compare it to manual user quantification. The software can be extended for other applications in meiosis research, such as incorporating machine learning approaches to categorize meiotic substages.
发表于 2025-3-23 03:51:34 | 显示全部楼层
Book 2024e topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls...Authoritative and cutting-edge, .Meiosis: Methods and Protocols. aims to ensure successful results in further studies of this vital field..
发表于 2025-3-23 06:58:13 | 显示全部楼层
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