Titlebook: Medulloblastoma; Methods and Protocol Abhinav Dey,Anshu Malhotra,Neha Garg Book 2022 The Editor(s) (if applicable) and The Author(s), under

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Childhood Medulloblastoma: An Overview,al advances in therapies over the last 20 years that have yielded a 5-year survival rate of 75%, high-risk patients (younger than 3 years, subtotal resection, metastatic lesions at diagnosis) still experience a 5-year overall survival of less than 70%. In this introductory chapter on pediatric MB, w

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Real-Time PCR Assays on Formalin-Fixed, Paraffin-Embedded Medulloblastomaspatients. However, much of its success depends on the quality and quantity of the starting material used for RNA extraction. Clinical samples are most often provided as formalin-fixed and paraffin-embedded, wherein the RNA is extensively degraded, affecting sensitivity. Here, we describe a real-time

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Validation of microRNA Target Genes Using Luciferase Reporter assay and Western Blot Analysisgenes enables the determination of their functional role in the cells. A single microRNA can target multiple genes, all of which have a microRNA binding site in their 3′ UTR. Putative target genes can be identified using target prediction software and gene expression analysis of microRNA expressing

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Analysis of Microarray Data from Medulloblastoma Tissue Samplesrrays that are printed with thousands of tiny spots in specified positions. Each spot contains a known DNA sequence or gene. These slides are commonly referred to as gene chips or DNA chips. The DNA molecules printed to each slide serve as probes to detect gene expression, which is also known as the

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Advancement of Single-Cell Sequencing in Medulloblastomaion of cells. The outward evolution of the technique from a manual method to the automation of single-cell sequencing is cogent. Lately, single-cell sequencing is widely used in various fields of science and has applications in neurobiology, immunity, cancer, microbiology, reproduction, and digestio

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Flow Cytometry Approaches to Obtain Medulloblastoma Stem Cells from Bulk Cultures. Medulloblastoma (MB), a primary CNS tumor, is a very fast-growing tumor affecting younger population. In order to characterize medulloblastoma cancer stem cells or studying the drug resistance in MB mediated through the cancer stem cells, it becomes essential to isolate and study them. Isolation a

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3D-on-2D: A Physiologically Relevant and Gel-Free In Vitro Coculture Method to Assay Antimetastatic chment of cells from the primary tumor followed by invasion through nearby blood vessels (Wong and Hynes. Cell Cycle 5(8):812–817, 2006). This is common to several solid tumors, including medulloblastoma (Van Ommeren et al. Brain Pathol 30:691–702, 2020). Because invasion is a crucial step in metast