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Titlebook: Mechanisms in Recombination; Rhoda F. Grell Book 1974 Plenum Press, New York 1974 DNA.Laboratory.biology.chromosome.energy.genes.molecule.

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书目名称Mechanisms in Recombination
编辑Rhoda F. Grell
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图书封面Titlebook: Mechanisms in Recombination; Rhoda F. Grell Book 1974 Plenum Press, New York 1974 DNA.Laboratory.biology.chromosome.energy.genes.molecule.
描述This book contains the papers presented at the Twenty-Seventh Annual Biology Division Research Conference which was held April 1-4, 1974 in Gatlinburg, Tennessee. The topic of the symposium was Mechanisms in Recombination and it follows by exactly twenty years the previous Gatlinburg Symposium on Genetic Recombination. During this interval, and the preceding years as well, the process of recombination has remained a central and tantalizing problem for geneticists. The subject assumes added significance with the recent appeal by a committee of leading scientists for a moratorium on the construction of certain types of recombinant molecules. That autonomously replicating molecules linking portions of pro­ karyotic and eukaryotic DNA can now be produced in vitro attests to the technical advances that have taken place in this field. Nevertheless, the details underlying the process in vivo continue to be elusive. This symposium brought together individuals studying recombi­ nation in organisms as widely separated as bacteriophage and mammals and using disciplinary approaches of comparable diversity. Conse­ quently the present volume summarizes much of current strategies and concepts con
出版日期Book 1974
关键词DNA; Laboratory; biology; chromosome; energy; genes; molecule; research
版次1
doihttps://doi.org/10.1007/978-1-4684-2133-0
isbn_softcover978-1-4684-2135-4
isbn_ebook978-1-4684-2133-0
copyrightPlenum Press, New York 1974
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Heterozygotes as Intermediates of Bacteriophage Recombinatione joint between the two parental contributions of a recombinant DNA molecule. Several lines of evidence demonstrate that heteroduplex regions, in which one strand of DNA is contributed by one of the parental molecules and its complementary strand by the other, are a prominent feature of the primary
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Recombination of Phage λ DNA ,at can be measured .. A major difficulty has been the lack of a direct and simple assay for recombinant DNA. I would like to describe conditions in which phage λ DNA recombines . and the recombinant DNA is assayed following its assembly into infectious λ particles.
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Transduction of ,B, Hosts is Promoted by λ ,, Functionls (see Signer, 1971, for a review). However, the . genes do not promote efficient specialized transduction of a .A. host (Manly ., 1969; Mizuuchi and Fukasawa, 1969; Gottesman ., 1974; H. Echols, personal communication). We report here that the . pathway does promote efficient transduction of .B. h
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Transformation and Transduction of , The Nature of Recombinants Formed by Rec, RecF, and λ Redhas recovered proficiency for recombination by virtue of a second suppressing mutation, . or .; and (2) the uptake of DNA is facilitated by treatment of the recipient cells with CaCl. (Oishi and Cosloy, 1972; Wackernagel, 1973). However, compared with other bacteria, the frequency of transformation
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