Titlebook: Measuring Calcium and Calmodulin Inside and Outside Cells; Ole H. Petersen Book 2001 Springer-Verlag Berlin Heidelberg 2001 Calcium.Fluore

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Measuring Ca2+ in the Nucleoplasm of Intact Cellsien (Tsien et al. 1982; Grynkiewicz et al., 1985), is that the protein does not permeate across cell membranes and thus needs to be microinjected. The technique was thus limited to large, sessile cells. The isolation of the aequorin cDNA (Inouye et al., 1985) completely changed this perspective. Ind

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their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.978-3-642-47737-9978-3-642-56851-0

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Measuring Calcium and Calmodulin Inside and Outside Cells

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Measuring Calcium and Calmodulin Inside and Outside Cells978-3-642-56851-0

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refines the approximate solution; and the final stage applies a partial lexicographic search procedure to generate an optimal solution. Extensive computational experiments show that our algorithms are able to solve large problems of size up to one million variables in less than 7 seconds CPU time on