Titlebook: Measurement of Ion Transport and Metabolic Rate in Insects; Timothy J. Bradley,Thomas A. Miller Book 1984 Springer-Verlag New York Inc. 19

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, Techniques for Studies of Malpighian Tubules,e formula 4/3.., where ., the radius of the drop, is measured by an eyepiece graticule fitted to a dissecting microscope. Such straightforward methods circumvent much of the experimental interference and the need for elaborate experimental chambers entailed when studying fluid or solute movements across a flat sheet of epithelial cells.

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Techniques for Studying Na+,K+-ATPase,ultaneous presence of Na. and K.. It was significant that Skou found that the monovalent cation-stimulated activity was abolished by ouabain, an inhibitor of active Na. transport (Schatzmann 1953). The enzyme was described as the Na.,K.-dependent ATPase (E.C.3.6.1.3.).

Patrimony

Methods for the Study of Transport and Control in Insect Hindgut, Sodium, potassium, chloride, water, amino acids, phosphate, and acetate are all reabsorbed from the rectal lumen into the hemocoel by active mechanisms (reviewed by Phillips 1980, 1981). A variety of preparations have been used in these studies, each having particular strengths and weaknesses.

Herbivorous

The Study of Atmospheric Water Absorption,id phases, find ways of measuring biological fluids of exceedingly high solute concentration and correspondingly reduced solvent activities, and frequently overcome the difficulties of working with very small animals.

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Spectrophotometry and Fluorometry in Ion Transport Epithelia,s . and even .. Their main advantages reside in being rapid and noninvasive, the latter allowing the optical monitoring to be performed in conjunction with other types of measurements normally used to assess epithelial function. Spectrophotometry and fluorometry of intact epithelia have provided novel information not obtainable by other methods.

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