| 书目名称 | Matrix Metalloproteinase Protocols | | 编辑 | Ian M. Clark | | 视频video | | | 概述 | Includes two comprehensive review chapters and twenty-five detailed method chapters.Provides state-of-the-art methodologies.Covers expression, purification, detection, and assay of MMPs and TIMPs.Deta | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Since the discovery of a collagen-degrading protease in the tadpole tail in 1962, matrix metalloproteinase research has led to the discovery of more than twenty distinct vertebrate MMPs, along with a variety of homologues from diverse organisms such as the sea urchin, plants, insects, and nematode worms. Fully updating and adding to the popular first edition, .Matrix Metalloproteinase Protocols, Second Edition. includes a series of state-of-the-art techniques provided by eminent experts in the field. Beginning with a brief overview of the MMP arena, from how these enzymes fit into the larger degradome to what occurs when their expression and function in the mouse is modulated, the volume continues with sections on the expression and purification of MMPs and TIMPs, the detection of MMPs and TIMPs at both the protein and mRNA level, and our ability to assay MMP and TIMP activities in a wide variety of circumstances. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooti | | 出版日期 | Book 2010Latest edition | | 关键词 | Escherichia coli; Microarray; Nucleotide; PCR; Proteomics; Single Nucleotide Polymorphism; enzymes; hybridi | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-60327-299-5 | | isbn_softcover | 978-1-4939-5622-7 | | isbn_ebook | 978-1-60327-299-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2010 |
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发表于 2025-3-21 17:20:19
