Titlebook: Mathematik für Wirtschaftswissenschaftler kompakt; Kurz und verständlic Franz Pfuff Textbook 2009 Vieweg+Teubner Verlag | Springer Fachmedi

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the important nucleic acid-based therapies to treat neurodegenerative diseases along with the opportunities and challenges depending on the disease and the drug target. In addition, we will also accentuate the present-day application of these inhibitory small RNAs and antisense oligonucleotides in a

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ol, it is important to elucidate how riboswitches interact with cognate and discriminate against non-cognate ligands. Here we outline the methodology to synthesize riboswitch RNAs and prepare riboswitch–ligand complexes for crystallographic and biochemical studies. The chapter describes how to desig

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g phase determination through molecular replacement of conserved Fab domains. Major steps described in this chapter include selection of a synthetic Fab library displayed on M13 phage against a structured RNA crystallization target, ELISA for initial choice of binding Fabs, Fab expression followed b

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reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical, .Nucleic Acid Chemistry: Methods and Protocols .seeks to aid scientists in the further study of detection for DNA and RNA..978-1-4939-6041-5978-1-62703-535-4Series ISSN 1064-3745 Series E-ISSN 1940-6029