| 书目名称 | Mass Spectrometry of Proteins and Peptides |
| 副标题 | Methods and Protocol |
| 编辑 | Mary S. Lipton,Ljiljana Paša-Tolic |
| 视频video | |
| 概述 | Comprehensive overview of latest mass spectrometric techniques for protein analysis.Step-by-step methods complete with detailed instructions for successful implementation.Written by established expert |
| 丛书名称 | Methods in Molecular Biology |
| 图书封面 |  |
| 描述 | When the last edition of this book was published in 2000, the field of proteomics was in its infancy. At that time, multidimensional liquid chromatographic separations were being introduced as an alternative to traditional gel-based techniques for separating complex protein and peptide mixtures prior to mass spectrometric detection. Today, this approach – referred to as shotgun proteomics – is considered routine for lar- scale global analyses of protein mixtures. Now in its adolescence, proteomics is fundamentally transforming biological and medical research. Much of this transformation can be attributed to technological advancements, particularly in mass spectrometry. Much wider accessibility of hi- resolution and mass measurement accuracy instrumentation in recent years has ini- ated a new revolution in the field by providing more reliable data and shifting the focus from cataloging proteins to precisely quantifying changes in protein abundance over time and in response to stimuli. Advanced mass spectrometers and novel ion d- sociation schemes such as electron transfer/capture dissociation make it possible to venture boldly into the maze of protein posttranslational modifications |
| 出版日期 | Book 2009Latest edition |
| 关键词 | Mass Spectrometry; Microarray; Proteomics; Termination; genes; membrane proteins; microorganisms; molecular |
| 版次 | 2 |
| doi | https://doi.org/10.1007/978-1-59745-493-3 |
| isbn_softcover | 978-1-62703-796-9 |
| isbn_ebook | 978-1-59745-493-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 |
| issn_series | 1064-3745 |
| copyright | Humana Press 2009 |
发表于 2025-3-21 18:45:08
