| 书目名称 | Mass Spectrometry and Genomic Analysis | | 编辑 | J. Nicholas Housby | | 视频video | | | 丛书名称 | Focus on Structural Biology | | 图书封面 |  | | 描述 | 76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometri | | 出版日期 | Book 2001 | | 关键词 | Allele; Base; DNA; Mass Spectrometry; Nucleotide; PCR; SNP; Single Nucleotide Polymorphism; Termination; gene | | 版次 | 1 | | doi | https://doi.org/10.1007/0-306-47595-2 | | isbn_softcover | 978-94-017-3909-2 | | isbn_ebook | 978-0-306-47595-5Series ISSN 1571-4853 Series E-ISSN 2542-9566 | | issn_series | 1571-4853 | | copyright | Springer Science+Business Media Dordrecht 2001 |
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发表于 2025-3-21 17:07:14
