Titlebook: Mass Cytometry; Methods and Protocol Helen M. McGuire,Thomas M. Ashhurst Book 2019 Springer Science+Business Media, LLC, part of Springer N

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书目名称Mass Cytometry影响因子(影响力)




书目名称Mass Cytometry影响因子(影响力)学科排名




书目名称Mass Cytometry网络公开度




书目名称Mass Cytometry网络公开度学科排名




书目名称Mass Cytometry被引频次




书目名称Mass Cytometry被引频次学科排名




书目名称Mass Cytometry年度引用




书目名称Mass Cytometry年度引用学科排名




书目名称Mass Cytometry读者反馈




书目名称Mass Cytometry读者反馈学科排名

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1064-3745 eagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Mass Cytometry: Methods and Protocols. aims to ensure successful results in the further study of this vital field..978-1-4939-9454-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for ctrophotometry and frequently exceeds 60%. Each custom-conjugated antibody is validated using positive and negative cellular control populations and is titrated for optimal staining at concentrations ranging from 0.1 to 10 μg/mL. The preparation of metal-labeled antibodies can be completed in 4.5 h, and titration requires an additional 3–5 h.

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Mass Cytometric Cell Cycle Analysisration of 5-Iodo-2′-deoxyuridine (IdU), combined with measurement of phosphorylated retinoblastoma protein (pRb), Cyclin B1, and phosphorylated Histone H3 (pHH3). These measurements can be integrated into a gating strategy that enables clear separation of all five phases of the cell cycle.

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Acquisition, Processing, and Quality Control of Mass Cytometry Data cell measurements requires specific considerations in acquiring and processing data. This chapter provides an overview of how to optimally acquire mass cytometry data and how to process this data for subsequent analysis and characterization of cell populations.