Titlebook: Marine Metagenomics; Technological Aspect Takashi Gojobori,Tokio Wada,Katsuhiko Mineta Textbook 2019 Springer Nature Singapore Pte Ltd. 201

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Metagenomic Methods: From Seawater to the Databasepecies during sampling. Here, we first describe methodology for a shallow river and in the later section methodology for a deep marine bay. In either case, slight modifications can be made to succeed in sampling. Determination of physicochemical parameters as metadata simultaneously is also an impor

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Collection of Microbial DNA from Marine Sediments, and grade of purification differ in each method and depending to sediment sample qualities. Various commercial kits for DNA extraction from environmental samples are available. A method appropriate to the purpose of the study should be selected.

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Primer Design, Evaluation of Primer Universality, and Estimation of Identification Power of Amplicon locus sequences of target taxa from public DNA databases, and designing primer set based on consensus sequence of retrieved sequences can be the solution for this problem. In this text, we provide methods and procedures to design universal primer pairs and to evaluate primer universality and identi

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High Coverage Expression Profiling (HiCEP) of Microbial Community Genomes in the Oceanting its methodological advantages in the analysis of metatranscriptome. In addition to high accuracy and reproducibility, this method can evaluate gene expression using either peak patterns or compositions of sequence clusters without annotation information, which enables us to highlight previously

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Seasonal Dynamics of Bacterial Community Composition in Coastal Seawater at Sendai Bay, Japanant correlation with water temperature, indicating seasonal fluctuation. Three OTUs significantly correlated with salinity, indicating that freshwater discharge influenced the bacterial community in the bay. Five OTUs that represented 38% of the total did not show any correlation with environmental

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