Titlebook: Mammary Stem Cells; Methods and Protocol Maria dM. Vivanco Book 2022Latest edition The Editor(s) (if applicable) and The Author(s), under e

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The Chicken Embryo Chorioallantoic Membrane (CAM): A Versatile Tool for the Study of Patient-Derivetly three-dimensional patient-derived xenograft (PDX) tumor modeling. We describe here a detailed method used to readily engraft established mouse PDX and primary patient tumor specimens on the CAM with as little as 25 mg of tissue per embryonated egg.

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Differential Proteomic Analysis of Complex Mixtures by Label-Free nLC MS/MS,dy in an unbiased manner..This chapter describes methods for sample preparation prior to mass spectrometry analysis. Besides, we describe a standard acquisition method, and some common bioinformatics analyses that help extracting biologically relevant information out of the achieved data.

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Intraductal Injections into the Mouse Mammary Gland,g which cells invade through the basement membrane. Either breast cancer cell lines or patient-derived breast cancer cells can be injected into the mammary duct using this protocol to model breast cancer progression. This protocol will cover the procedures required to perform this technique.

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Lucia Garcia del Valle,Marta G. Montero,Martin Jechlinger