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Titlebook: MERS Coronavirus; Methods and Protocol Rahul Vijay Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 SARS-CoV.ME

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发表于 2025-3-21 18:03:35 | 显示全部楼层 |阅读模式
书目名称MERS Coronavirus
副标题Methods and Protocol
编辑Rahul Vijay
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: MERS Coronavirus; Methods and Protocol Rahul Vijay Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 SARS-CoV.ME
描述.This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quantitation of virus and anti-viral factors, and mouse models for MERS -coronavirus.   Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... Authoritative and cutting-edge, .MERS Coronavirus: Methods and Protocols. aims to ensure successful results in the further study of this vital field..
出版日期Book 2020
关键词SARS-CoV; MERS-CoV; metagenomics; reverse genetics; ; cDNA clones
版次1
doihttps://doi.org/10.1007/978-1-0716-0211-9
isbn_softcover978-1-0716-0213-3
isbn_ebook978-1-0716-0211-9Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
The information of publication is updating

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Evaluating MERS-CoV Entry Pathwaysods, highlighting virus-cell entry factors, entry inhibitors, and viral determinants that specify the cell entry routes. While the specific methods described herein were utilized to reveal MERS-CoV entry pathways, they are equally suited for other CoVs, as well as other protease-dependent viral species.
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Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytells allowing expression of the spike protein of the highly pathogenic Middle East respiratory syndrome coronavirus in the presence or absence of a cellular trypsin-like transmembrane serine protease, matriptase. Such analysis enables the characterization of cleavage patterns produced by a host protease on a coronavirus spike glycoprotein.
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1064-3745 ation advice from the experts.This volume provides various techniques and methodologies currently used in the study of MERS-CoV. Chapters are divided into four parts detailing evolution and entry of MERS-coronavirus, genetic alteration and structural determination of MERS-coronavirus proteins, quant
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Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in inment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species.
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