Titlebook: Lung Innate Immunity and Inflammation; Methods and Protocol Scott Alper,William J. Janssen Book 2018 Springer Science+Business Media, LLC,

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Isolation and Characterization of Mouse Fibroblasts matrix and are important in the maintenance of alveolar epithelial cells. To understand the role of fibroblasts both in homeostasis and disease, we isolate fibroblasts and grow them in culture. Two methods are presented here for the isolation and maintenance of mouse primary lung fibroblasts.

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Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells Uenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). To study the alveolar epithelial barrier function under pathological conditions, we developed an in vitro model of acute lung injury using cultured human alveolar epithelial type II (ATII) cells. Here we describe the m

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Measuring Neutrophil Bactericidal Activity. Regardless of the mechanism, the ability to kill pathogens is a vital outcome. This chapter describes a method to measure the in vitro bactericidal activity of isolated neutrophils as the endpoint of converging innate immune functions.

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