| 书目名称 | Low-Cost Methods for Molecular Characterization of Mutant Plants |
| 副标题 | Tissue Desiccation, |
| 编辑 | Bradley J. Till,Joanna Jankowicz-Cieslak,Bernhard |
| 视频video | http://file.papertrans.cn/589/588845/588845.mp4 |
| 概述 | Open Access provided with a grant from the International Atomic Energy Agency.Describes low-cost and easy-to-use assays for the molecular characterization of mutant plant material.The experimental pro |
| 图书封面 |  |
| 描述 | .This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.. |
| 出版日期 | Book‘‘‘‘‘‘‘‘ 2015 |
| 关键词 | Crop improvement; DNA extraction; Induced mutations; Low-cost assays; Mutant germplasm characterization; |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-319-16259-1 |
| isbn_softcover | 978-3-319-38667-6 |
| isbn_ebook | 978-3-319-16259-1 |
| copyright | The Editor(s) (if applicable) and the Author(s) 2015 |
|Archiver|手机版|小黑屋|
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