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Titlebook: Lotus japonicus Handbook; Antonio J. Márquez Book 2005 Springer Science+Business Media B.V. 2005 Assimilat.DNA.Expression.RNA.enzymes.gene

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Drought and saline stress species. Up to 12-fold proline accumulation was detected in . plants in relation to drought and salt stress treatments. In parallel to the accumulation of proline as an osmolyte, the above mentioned stress conditions produced in . an increased level of oxidative damage as evaluated through measurem
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A procedure for , nodulation studiesnvestigating the different stages of the Rhizobium-legume interaction. A standard . procedure can facilitate analysis of the kinetics of phenotype at the molecular level occurring after Rhizobium infection. This is essential when comparing, for instance, the expression pattern of nodulin or marker g
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Arbuscular mycorrhizaThe roots of almost all vascular plants can interact with AM fungi with the notable exception of many brassicas, including the model plant .. Legumes, which also form symbiotic nitrogen-fixing nodules, have been intensively studied over the past decade, revealing a surprising genetic overlap between
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Concurrent visualization of , and , reporter gene expressionguish between the sites of expression of plant and bacterial genes, respectively, in symbiotic tissues. These conditions involve the fixation of cytoplasmic proteins with cold methanol before detecting . expression, and the addition of detergents to the incubation solution in order to inactivate end
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Embedding root and nodule tissue in plastic (BMM)ion on serial 5–8 μm sections is most frequently performed with tissue embedded in paraffin wax but it is often difficult to obtain high-resolution sections from soft tissue with this embedding process. To overcome this, alternative localisation protocols have been developed utilising plastic resins
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96-Well DNA isolation methodp based cloning or any application which requires long term DNA storage. The protocol has been designed for DNA extraction from leaf material, preferably young leaf tissue should be used as this minimises samples being contaminated with polysaccharides and phenolics.
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