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Titlebook: Listeria monocytogenes; Methods and Protocol Kieran Jordan,Edward M. Fox,Martin Wagner Book 2014 Springer Science+Business Media New York 2

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书目名称Listeria monocytogenes
副标题Methods and Protocol
编辑Kieran Jordan,Edward M. Fox,Martin Wagner
视频video
概述Includes cutting-edge methods and protocols to study Listeria monocytogenes.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the expert
丛书名称Methods in Molecular Biology
图书封面Titlebook: Listeria monocytogenes; Methods and Protocol Kieran Jordan,Edward M. Fox,Martin Wagner Book 2014 Springer Science+Business Media New York 2
描述.Listeria monocytogenes is still a major threat to public health. A new book in the .Methods in Molecular Biology. series, .Listeria monocytogenes: Methods and Protocols. addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of .Listeria. at a molecular level. The topics covered include sampling in order to isolate .Listeria., methods for their identification and characterization, methods for gene manipulation and finally, methods for control of the organism. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Vital and authoritative, .Listeria monocytogenes: Methods and Protocols. aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research..
出版日期Book 2014
关键词Bacterium sampling; Control methods; Gene manipulation; L; monocytogenes; Pathogen; Strain manipulation
版次1
doihttps://doi.org/10.1007/978-1-4939-0703-8
isbn_softcover978-1-4939-5436-0
isbn_ebook978-1-4939-0703-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
The information of publication is updating

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Multilocus Sequence Typing (MLST) of ,r methods as it can reconstruct ancestral and evolutionary linkage between . isolates. MLST detects all genetic variations within the amplified housekeeping gene that accumulate slowly. This chapter describes how to undertake MLST.
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Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of ,ated to specific double-stranded adaptors. The ligated fragments are then amplified by PCR using fluorescently labelled primers. Fluorescent amplified fragments are separated by size on an automated sequencer with a size standard. fAFLP is a rapid, highly reproducible technique which can be used to discriminate and subtype . strains.
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Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Method. For mutagenesis in ., we describe two different plasmid-based approaches, which facilitate the introduction of this spliced amplicon in place of the corresponding segment of chromosomal DNA: the pKSV7 system and the pORI280/pVE6007 system.
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Sampling the Processing Environment for ,to sample, the best time for sampling and the frequency of sampling are also given. Such details help hygienists/microbiologists to be successful in their attempts to isolate strains of ., even if such bacteria are well attached to surfaces or located in niches that are difficult to reach.
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Extraction and Analysis of Plasmid DNA from ,ient for analytical purposes but may be upscaled for further downstream analysis. Electrophoretic separation of the resultant lysate allows conclusions to be made on the presence, number, copy number, and size of the plasmids in the analyzed bacterial strains.
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