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Titlebook: Lentivirus Gene Engineering Protocols; Maurizio Federico Book 20031st edition Humana Press 2003

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Small- to Large-Scale Production of Lentivirus Vectorsiginally established for the production of retroviral vectors based on Moloney murine leukemia (MoMLV) virus (.). Simian virus 40 (SV40)-transformed African green monkey kidney (COS-7) cells (.) and human TE671 rhabdomyosarcoma cells (.) have also been used to generate human immunodeficiency virus t
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Detection and Titration of Lentivirus Vector Preparationstential gene transfer applications for any viral vector of interest. This accuracy is especially critical if anticipated clinical applications will require scale-up of vector production or if multiple different vector stocks will be required for serial transductions of target cell populations over t
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Detection of Replication-Competent Lentiviral Particles but have also raised safety concerns because of potential creation of replication-competent lentivirus (RCL) by uncontrolled recombination (.–.). In order to be safe for clinical use, vector preparations must be formally and extensively tested to show they are absolutely free of RCL. Various method
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