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Titlebook: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools; Maurizio Federico Book 2016 Springer Science+Business Media New York 2

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书目名称Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools
编辑Maurizio Federico
视频videohttp://file.papertrans.cn/585/584969/584969.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools; Maurizio Federico Book 2016 Springer Science+Business Media New York 2
描述.This volume provides current methods and protocols for geneand protein delivery based on both lentivirus-generated and spontaneouslyreleased nanovesicles.  .Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools .guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful .Methods in Molecular Biology .series format, chaptersinclude introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and cutting-edge, .Lentiviral Vectors andExosomes as Gene and Protein Delivery Tools. aims to ensure successfulresults in the further study of this vital field..
出版日期Book 2016
关键词cell engineering; LV-producer cells; hematopoietic stem cells; spermatozoa; hepatocytes
版次1
doihttps://doi.org/10.1007/978-1-4939-3753-0
isbn_softcover978-1-4939-8129-8
isbn_ebook978-1-4939-3753-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Production and Concentration of Lentivirus for Transduction of Primary Human T Cells expensive. We describe a method for generating lentivirus that is simple to perform and does not require the purchase of non-standard equipment to transduce primary human T cells. Therefore, we provide an optimized protocol that is easy to implement and allow transduction with high efficiency and reproducibility.
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Book 2016cles.  .Lentiviral Vectors and Exosomes as Gene andProtein Delivery Tools .guides readers through methods on macromoleculedelivery and chapters describe the LV-based protocols of gene engineering. Written in thehighly successful .Methods in Molecular Biology .series format, chaptersinclude introduct
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Armory
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Conditional RNAi Using the Lentiviral GLTR Systemiviral vectors for conditional shRNA expression cassette delivery co-express additional genes that allow (1) the use of fluorescent proteins for color-coded combinatorial RNAi or monitoring RNAi induction (pGLTR-FP), (2) selection of transduced cells (pGLTR-S), and (3) the generation of conditional cell lines using a one-vector system (pGLTR-X).
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