Titlebook: Leishmania; Methods and Protocol Joachim Clos Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Neglected Tropic

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Host-Free Systems for Differentiation of Axenic ,fic medium. Shifting promastigotes to this medium, followed by incubation in a CO. incubator, induced differentiation. Axenic amastigotes reach maturation within 5 days, resembling the time it takes in vivo. This chapter provides a complete protocol that should be useful for both Old and New World species of ..