Titlebook: Legionella; Methods and Protocol Carmen Buchrieser,Hubert Hilbi Book 2019Latest edition Springer Science+Business Media, LLC, part of Sprin

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Selection of , Virulence-Related Traits by Environmental Protozoareplicate within both amoebae and mammalian macrophages. Here we describe how to isolate environmental protozoa that prey on virulent .. These protists are extremely useful models to study the cellular mechanisms employed by . to survive and grow in its natural environment. We present here procedure

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Migration of , Through , Biofilmshe environment . forms and colonizes biofilms, which usually comprise complex multispecies communities. In these biofilms . persists and replicates intracellularly in protozoa, such as the amoeba .. The interactions between sessile . in biofilms and their natural protozoan hosts are not understood o

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Scar-Free Genome Editing in ools have been developed to achieve this goal in a lot of bacteria ., and a major key is to be able to manipulate the targeted genome region with a minimum impact on the rest of the genome. Here, we described a two-step protocol relevant in .. This efficient two-step protocol uses the natural transf

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Genetic Manipulation of Non-,: Protocols Developed for % of clinical disease worldwide. However, in certain geographical regions, infections with non-. species are responsible for a significant proportion of diagnosed Legionnaires’ disease. Understanding the pathogenesis of these non-. species of . is an important step toward clinical intervention. The

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