Titlebook: Laser Scanning Microscopy and Quantitative Image Analysis of Neuronal Tissue; Lidia Bakota,Roland Brandt Book 2014 Springer Science+Busine

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Translation, Touch, and Overlap in Multi-fluorescence Confocal Laser Scanning Microscopy to Quantitresolution (the optical component) and pixel resolution (the computer component) are extremely important issues. Microscope resolution depends on refraction inherent in the selected objective lens. The refractive properties and therefore the resolution of a lens are expressed by its numerical apertu

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Surgical Procedures to Study Microglial Motility in the Brain and in the Spinal Cord by In Vivo Twoegenerative diseases. The processes of microglia are continuously moving to survey the brain parenchyma. Depending on local signals, microglia can almost instantaneously change their morphology. Two-photon laser-scanning microscopy (2P-LSM) has become the method of choice to study microglia in vivo.

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Combining Multichannel Confocal Laser Scanning Microscopy with Serial Section Reconstruction to Anamens without sectioning. However, technical limits still hamper the use of these approaches to investigate deep anatomical regions. The alternative to intact structures imaging is serial section reconstruction, but this technique requires a high number of sections to acquire and register in order to

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Modeling Excitotoxic Ischemic Brain Injury of Cerebellar Purkinje Neurons by Intravital and In Vitrtral nervous system (CNS) caused, for example, by stroke or neurodegenerative pathologies. Several experimental models have been established over the years to study structural changes under these conditions using both in vitro and in vivo approaches. In this context, the use of transgenic reporter m

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Quantitative Analysis of Axonal Outgrowth in Mice,of precise peripheral networks. Interactions of growing axons with their environment and co-extending nerve fibers have been shown to critically contribute to the establishment of neuronal projections to the limb, governing accurate fasciculation of heterotypic fiber systems and mediating the stereo

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Analysis of Actin Turnover and Spine Dynamics in Hippocampal Slice Cultures, been described between activity-dependent synaptic plasticity and structural plasticity in the CNS. Actin is highly enriched within spine heads, and changes in actin cytoskeleton dynamics have been show to mediate changes in the size and shape of spines. However, most studies so far either analyzed

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