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Titlebook: Laboratory Diagnosis of Infectious Diseases Principles and Practice; VOLUME II Viral, Ric E. H. Lennette,P. Halonen,W. J. Hausler Book 1988

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Immunoreagentsfluenza, paramyxo-, reo-, and rhinoviruses.. . Antisera and viral antigens.. . Production and selection of hybridoma; influence of immunoassay on antibody specificity.. . Types of antigenic determinants in viruses.. . Purification of virions, preparation of viral proteins by dissociation of virions
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Electron Microscopyding direct application, water drop, agar diffusion, pseudoreplica, ultracentrifugation... Preparation of thin sections by standard and rapid embedding methods; collection of cell suspensions by hematocrit centrifugation... Negative staining methods, including direct IEM, solid phase IEM, serum-in-a
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Nucleic Acids in Viral Diagnosiscarrying a label.. . Exquisite specificity, sensitivity of 105 for target nucleic acid molecules.. . Easy specimen pretreatment and easy separation of hybrid from unreacted probe.. . Sandwich hybridization is a three-component reaction with a capture probe attached on a solid matrix, and a labeled p
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Virus Taxonomy and Nomenclatureroupings useful in the diagnostic setting... “Differential diagnosis” and “rule-out diagnosis,” causal relationship between virus and disease, the adequate description of new viruses... Family characteristics and listings of member viruses.
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Epstein-Bair Virustor in BL and NPC... Human saliva and blood... Acute or insidious onset with fever, pharyngitis, lymphadenopathy. May be associated with varying degrees of splenomegaly, hepatomegaly, or frank hepatitis. Rare central nervous system involvement... Marked lymphocytosis involving most of the lymphoreti
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Cytomegalovirus (HCMV), a member of the herpesvirus family... Human, by 1) direct contact, including sexual and parturition; 2) vertical; 3) breast milk; 4) blood or organ transplantation... Clinical manifestations depend on the syndrome... hepatosplenomegaly, petechial rash, microcephaly, intracerebral calcificat
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Varicella-Zoster Virustact or airborne transmission... Acute onset and progression of rash, leading to vesicles is most common feature of varicella. A wide variety of sequelae, including purpura, arthritis, pneumonitis, and involvement of the central nervous system, may occur in rare in-stances. In herpes zoster, eruptio
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